Detection of cysteine residue numbers in wheat gluten proteins by MALDI-TOF
نویسندگان
چکیده
منابع مشابه
Characterization of wheat gluten proteins by HPLC and MALDI TOF mass spectrometry.
We have performed a detailed characterization and identification of wheat gluten proteins obtained from the Teal variety of Canadian hard red spring wheat. RP-HPLC separation of the sample into 35 fractions has reduced the spectral complexity; this was followed by MALDI mass spectrometry (MS), which showed the presence of six or fewer resolved protein components above 20 kDa in each RP-HPLC fra...
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متن کاملImproved identification of wheat gluten proteins through alkylation of cysteine residues and peptide-based mass spectrometry
The concentration and composition of wheat gluten proteins and the presence, concentration and location of cysteine residues therein are important for wheat flour quality. However, it is difficult to identify gluten proteins, as they are an extremely polymorphic mixture of prolamins. We here present methods for cysteine labeling of wheat prolamins with 4-vinylpyridine (4-VP) and iodoacetamide (...
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ژورنال
عنوان ژورنال: International Journal of Mass Spectrometry
سال: 2015
ISSN: 1387-3806
DOI: 10.1016/j.ijms.2015.09.014